New Paper - A02 Bauer
27.11.2024
New publication from Stefan Bauer's group
Nathanael Kegel, Stefan's PhD student on the TRR237 project just published his first first author publication - congratulations!
Cationic lysosomotropic molecules such as ammonium salts and chloroquine inhibit influenza A virus (IAV) infection in cell culture by counteracting endosomal acidification and hampering viral-endosomal fusion. Here, we studied the effects of storage of L-glutamine-supplemented cell culture media on accumulation of ammonium and inhibition of IAV infection. The storage-related inhibitory effect was observed for DMEM and OptiMEM media but not for RPMI medium, and was more pronounced for IAV with pH-stable hemagglutinin. Our results highlight the importance to consider potential presence of virus-inhibiting lysosomotropic agents and/or their production in culture media with negative effect on influenza virus infection.
published in Virology: Decomposition of L-glutamine and accumulation of ammonium in cell culture media inhibit infectivity of influenza viruses
