A30 - Higher-order structures of ligand-bound RIG-I and cGAS
The cytosolic receptors RIG-I and cGAS detect invading virus RNA or DNA. While RIG-I recognizes short (20-1000 bp) 5’-triphosphorylated double stranded(ds) RNA, cGAS detects either dsDNA longer than 40-60 bp or short Y-form dsDNA containing unpaired guanosines flanking short (12 to 20 bp) dsDNA (YSD). Upon ligand binding, RIG-I/cGAS form higher-order complexes that activate kinases. Quaternary complex structures of full-length RIG-I/cGAS with short ligands are elusive so far. Using cryo-EM, structure guided mutational analysis, immunological, biochemical and filament-elongation assays we aim at resolving the mechanism behind the activation of cGAS and RIG-I by YSD and short RNA ligands, respectively.
Prof. Dr. Matthias GeyerUniversitätsklinikum Bonn, Universität Bonn +49 (0)228 287-51400 External web links: |
Prof. Dr. Martin SchleeUniversitätsklinikum Bonn, Universität Bonn +49 (0)228 287-16080 External web links: |
